| John Syclyng | 1505 - 1506 | 1st Master |
| Richard Wyatt | 1506 - 1508 | 2nd Master |
| Thomas Thompson | 1508 - 1517 | 3rd Master |
| John Watson | 1517 - 1531 | 4th Master |
| Henry Lockwood | 1531 - 1548 | 5th Master |
| Richard Wilkes | 1548 - 1553 | 6th Master |
| Cuthbert Scot | 1553 - 1556 | 7th Master |
| William Tayler | 1556 - 1559 | 8th Master |
| Edward Hawford | 1559 - 1582 | 9th Master |
| Edmund Barwell | 1582 - 1609 | 10th Master |
| Valentine Cary | 1609 - 1622 | 11th Master |
| Thomas Bainbridge | 1622 - 1646 | 12th Master |
| Samuel Bolton | 1646 - 1654 | 13th Master |
| Ralph Cudworth | 1654 - 1688 | 14th Master |
| John Covel | 1688 - 1723 | 15th Master |
| William Towers | 1723 - 1745 | 16th Master |
| George Rooke | 1745 - 1754 | 17th Master |
| Hugh Thomas | 1754 - 1780 | 18th Master |
| John Barker | 1780 - 1808 | 19th Master |
| Thomas Browne | 1808 - 1814 | 20th Master |
| John Kaye | 1814 - 1830 | 21st Master |
| John Graham | 1830 - 1849 | 22nd Master |
| Joseph Shaw | 1849 | 23rd Master |
| James Cartmell | 1849 - 1881 | 24th Master |
| Charles Swainson | 1881 - 1887 | 25th Master |
| John Peile | 1887 - 1910 | 26th Master |
| Sir Arthur Shipley | 1910 - 1927 | 27th Master |
| Norman McLean | 1927 - 1936 | 28th Master |
| Sir Charles Darwin | 1936 - 1939 | 29th Master |
| Charles Raven | 1939 - 1950 | 30th Master |
| Brian Downs | 1950 - 1963 | 31st Master |
| The Lord Todd | 1963 - 1978 | 32nd Master |
| Sir John Plumb | 1978 - 1982 | 33rd Master |
| Sir Hans Kornberg | 1982 - 1995 | 34th Master |
| Alan Munro | 1995 - 2002 | 35th Master |
| Malcolm Bowie | 2002 - 2006 | 36th Master |
| Frank Kelly | 2006 - 2016 | 37th Master |
| Jane Stapleton | 2016 - 2022 | 38th Master |
| The Lord McDonald of Salford | 2022 | 39th Master |